Genetic Dna And Bioinformatics ( Accession No. Xp Essay

RESULTS Isolation of BbovM17LAP gene The BbM17LAP gene available in GenBank (accession no. XP_001609968) was accessed through the National Center for Biotechnology Information (NCBI), and its sequence was retrieved for further analysis. The genomic DNA of BbM17LAP was found in chromosome 2, extending between 1,045,409 bp and 1,047,164 bp of the genome. The ORF of the corresponding mRNA encoding BbM17LAP consists of 1,578 bp. Alignment of the mRNA sequence with genomic DNA using Genetyx revealed that the gene has 4 introns within its ORF. The first one was a 33- bp intron (50-ggtatgtttgtttgaatactgctgaatctgtgta-30) situated near the 50 end of the gene. The other nucleotide sequences included a 35-bp (50-agatacttacagtaacgatacaagttcatgtaggt-30) intron, a 34-bp (50-tgggttacactctttatgctatatttgttatagg-30) intron, and a 40-bp (50- gtaagttaattgggctacaagaagcgtaatatacatacag-30) intron all located close to each other near the 30 end. Isolation of BbovM17LAP cDNA and bioinformatics analysis A pair of primers targeting the ORF of BbM17LAP was used to amplify and sequence the gene from a B. bovis cDNA expression library. This BbM17LAP cDNA encoded a predicted 55-kDa protein without a signal peptide. The translated BbM17LAP polypeptide shared significant homology with B. gibsoni M17LAP, having 71% amino acid identity and similarity (E value ¼0). The other significant homologues included Theileria parva and Theileria annulata M17 LAPs, both having 61% amino acid identities. The polypeptide